Journal: Cells

Article Title: Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population

doi: 10.3390/cells9081885

Figure Lengend Snippet: HIF-1α expressions in different cybrids under hypoxia-ischemia. Comparison of HIF-1α expression under hypoxic-ischemic conditions in cybrids harboring common mitochondrial haplogroups found in the ethnic Chinese population (B4, B5, D4, D5, F1, F2, N9). The 143B cybrid was used to represent the Caucasian population. Actin was used as a loading control. Data represent the mean ± SD of at least three independent experiments ( # p < 0.005). N was used to represent normoxic and H to represent the hypoxic condition for 24 h.

Article Snippet: MtDNA-depleted ρ0 cells were established by the treatment of human osteosarcoma 143B cells (BCRC 60439) (Bioresource Collection and Research Center, Taipei, Taiwan) with ethidium bromide for three months, and illustration of their mtDNA-less cellular status by RT-PCR has been previously described [ , ].

Techniques: Comparison, Expressing, Control

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: The infiltration profile of different immune cells in TME for men and women with osteosarcoma. The analysis was performed using (A) CIBERSORT, (B) MCP‐counter, (C) ssGSEA, and (D) Xcell computational method

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques:

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Consistent clustering diagram of immune cells in different clusters. A heatmap showing the distribution of immune genes (A), and four subgroups of CDF (B). (C) A heatmap showing the infiltration level of immune cells in the four osteosarcoma subgroups. The clusters were generated based on TP, ISs, and SSs

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Generated

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Box plot displaying the expression pattern of immune‐related genes between osteosarcoma subgroups. (A and B) ISs and SSs for the four osteosarcoma clusters. Compared with the other three clusters, cluster 4 had a higher immune score. (C–F) The expression levels of multiple immune genes among the four clusters. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ****, p < 0.0001

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Expressing

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Distribution of the main pathological osteosarcoma subtypes in the four osteosarcoma clusters. (A–D) The major pathological subtypes including Leiomyosarcoma (LMS), Myxofibrosarcoma (MF), undifferentiated pleomorphic sarcoma (MFH), and Undifferentiated Pleomorphic Sarcoma (UPS) in the four clusters. (E and F) The survival rate of patients in different subtypes

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques:

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Validation of NPTX2 function in osteosarcoma. (A–C) Association of NPTX2 mRNA with CD8A, IFNG, and GZMB expression in osteosarcoma tissues ( n = 35). (D) The knockdown efficacy of NPTX2 in HOS and SW1353 cells as determined by qPCR. (E and F) Cell proliferation was measured with the CCK8 assays in HOS and SW1353 cells transfected with siRNAs or NC. (G and H) Apoptosis of HOS cells and SW1353 cells after transfection with siRNAs or NC was detected by flow cytometric assay. (I and J) The expression of CCL4, CCL5, CXCL9, CXCL10, and CXCL13 as measured with qPCR in HOS‐NPTX2‐NC and HOS‐NPTX2‐siRNAs cells, or SW1353‐NPTX2‐NC and SW1353‐NPTX2‐siRNAs cells. (K and L) The expression of CCL4, and CXCL13 as quantified with ELISA assay. (M and N) Association of NPTX2 mRNA with CCL4 expression in the TCGA datasets and in osteosarcoma tissues from our patients ( n = 35). (O and P) Association of CCL4 mRNA with CD8A expression in the TCGA datasets and osteosarcoma tissues from our patients ( n = 35)

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Biomarker Discovery, Expressing, Knockdown, Transfection, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Hub genes for the osteosarcoma prognostic model. (A) Multivariate cox regression analysis of hub genes. (B) Kaplan–Meier curves showing the OS of patients with high and low prognostic scores. (C) The time‐dependent ROC curves displaying the prognostic prediction of the hub genes model

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques:

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Relationship between clinical features and prognosis of osteosarcoma. (A) Multivariate cox regression analysis results indicating the relationship between metastasis, risk score, and OS. (B) The ROC showing the prognostic accuracy of the 11‐gene model across several cancer and disease factors (gender, across age, in metastatic, and non‐metastatic tumors as well as depth, length, and width). (C) The predictive accuracy of the 11‐gene risk score model in predicting immunotherapy response. The low‐risk group had better OS than the high‐risk group ( p < 0.0001)

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: